DNA

Part:BBa_K2100054

Designed by: Julia Goupil   Group: iGEM16_MIT   (2016-10-19)


Sensor 18A

This part contains two main transcriptional units. One encoding hef1a-mKate adjacent to 4 miRNA target sites. The other encodes hef1a-BFP. mKate production signifies the amount of repression occurring due to miRNA activity. BFP can be used as transfection marker.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 769
    Illegal EcoRI site found at 1422
    Illegal EcoRI site found at 2651
    Illegal EcoRI site found at 3447
    Illegal EcoRI site found at 7160
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 8340
    Illegal XbaI site found at 8346
    Illegal SpeI site found at 5285
    Illegal PstI site found at 845
    Illegal PstI site found at 1128
    Illegal PstI site found at 1777
    Illegal PstI site found at 2282
    Illegal PstI site found at 3984
    Illegal PstI site found at 8325
    Illegal PstI site found at 8636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 769
    Illegal EcoRI site found at 1422
    Illegal EcoRI site found at 2651
    Illegal EcoRI site found at 3447
    Illegal EcoRI site found at 7160
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 8340
    Illegal SpeI site found at 5285
    Illegal PstI site found at 845
    Illegal PstI site found at 1128
    Illegal PstI site found at 1777
    Illegal PstI site found at 2282
    Illegal PstI site found at 3984
    Illegal PstI site found at 8325
    Illegal PstI site found at 8636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 769
    Illegal EcoRI site found at 1422
    Illegal EcoRI site found at 2651
    Illegal EcoRI site found at 3447
    Illegal EcoRI site found at 7160
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 8340
    Illegal BglII site found at 192
    Illegal BglII site found at 2031
    Illegal BglII site found at 3527
    Illegal BglII site found at 7868
    Illegal BglII site found at 9600
    Illegal BamHI site found at 10254
    Illegal XhoI site found at 727
    Illegal XhoI site found at 2430
    Illegal XhoI site found at 7116
    Illegal XhoI site found at 9999
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 769
    Illegal EcoRI site found at 1422
    Illegal EcoRI site found at 2651
    Illegal EcoRI site found at 3447
    Illegal EcoRI site found at 7160
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 8340
    Illegal XbaI site found at 8346
    Illegal SpeI site found at 5285
    Illegal PstI site found at 845
    Illegal PstI site found at 1128
    Illegal PstI site found at 1777
    Illegal PstI site found at 2282
    Illegal PstI site found at 3984
    Illegal PstI site found at 8325
    Illegal PstI site found at 8636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 769
    Illegal EcoRI site found at 1422
    Illegal EcoRI site found at 2651
    Illegal EcoRI site found at 3447
    Illegal EcoRI site found at 7160
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 8340
    Illegal XbaI site found at 8346
    Illegal SpeI site found at 5285
    Illegal PstI site found at 845
    Illegal PstI site found at 1128
    Illegal PstI site found at 1777
    Illegal PstI site found at 2282
    Illegal PstI site found at 3984
    Illegal PstI site found at 8325
    Illegal PstI site found at 8636
    Illegal NgoMIV site found at 930
    Illegal NgoMIV site found at 1213
    Illegal NgoMIV site found at 2165
    Illegal NgoMIV site found at 8551
    Illegal NgoMIV site found at 8832
    Illegal NgoMIV site found at 9734
    Illegal AgeI site found at 1543
    Illegal AgeI site found at 8680
    Illegal AgeI site found at 9112
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7139
    Illegal BsaI site found at 11026
    Illegal BsaI.rc site found at 104


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Categories
Parameters
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